: Figure Out the cycle at which the next derivative of fluorescence with regard to cycle selection is maximal (Rasmussen, 2001). Local fitting to a parabola could be used to define the peak position with sub‐cycle precision. Bacterial proteins, damaged cell walls, and denatured chromosomal DNA enmeshed into big complexes https://www.facebook.com/NewTechFind/posts/pfbid02xbHY6Ab6tAcbzj3yzR33nmkjaR9ojeezsGUFyFJhNCvpx8SsvUPu3QUPLXmsPmtil