Fluorescence labeling of bacterial pathogens has a broad range of interesting applications including the observation of living bacteria within host cells. We constructed a novel vector based on the E. coli streptococcal shuttle plasmid pAT28 that can propagate in numerous bacterial species from different genera. https://www.ealisboa.com/super-save-Edible-Crotchless-Gummy-Panty-Peach-limited-grab/