With an internal transcribed spacer of 18 S. 5. 8 S and 26 S nuclear ribosomal DNA (nrDNA ITS) as DNA marker. we report a colorimetric approach for authentication of Pseudostellaria heterophylla (PH) and its copyright species based on the differentiation of the nrDNA ITS sequence. https://megavitamines.shop/product-category/vitamin-k/